Recombinant Human Neurotrophin-4
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
NT-4 也称为 NT-5，是一种神经元和上皮生长因子，属于 NGF-β 家族。NT-4 前体由 24 a.a. 信号肽、56 a.a. 和 130 a.a. NT-4 组成。成熟蛋白有六个Cys氨基酸残基，与NT-3、BDNF具有相对结构（共享约48%-52%的序列同一性）。此外，它与小鼠和大鼠 NT-4 共享 91% 和 95% 的 a.a. 序列同一性。NT-4 主要在前列腺中表达，胸腺、胎盘和骨骼肌水平较低。它可以与LNGFR和trkB受体结合，在调节生存和维持周围感觉交感神经元中起着至关重要的作用。NT-4 缺陷可能导致原发性 1O 型开角型青光眼。
Reference
1. Gao WQ, Zheng JL, Karihaloo M. 1995. J Neurosci, 15: 2656-67
2. Ogborn DIandGardiner PF. 2010. Muscle Nerve, 41: 385-91
3. Peinado-Ramon P, Salvador M, Villegas-Perez MP, et al. 1996. Invest Ophthalmol Vis Sci, 37: 489-500
4. Yuen ECandMobley WC. 1999. Exp Neurol, 159: 297-308
5. Sakuma K, Watanabe K, Sano M, et al. 2001. Brain Res, 907: 1-19.
| Gene ID | 4909 |
| Accession # | P34130 |
| Alternate Names | Neurotrophin-5, NT-5 |
| Source | Escherichia coli. |
| M.Wt | Approximately 28.1 kDa, a noncovalently linked homodimer of two 14.0 kDa polypeptide monomers (262 total amino acid residues). |
| AA Sequence | MGVSETAPAS RRGELAVCDA VSGWVTDRRT AVDLRGREVE VLGEVPAAGG SPLRQYFFET RCKADNAEEG GPGAGGGGCR GVDRRHWVSE CKAKQSYVRA LTADAQGRVG WRWIRIDTAC VCTLLSRTGR A |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 5.5. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 104 IU/mg. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
质量控制和DataSheet
- 批次:
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纯度 > 97 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rHuNT-4 as determined by LAL method.