Recombinant Human MIP-4/CCL18
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
人类 CCL18 由位于 17 号染色体上的 CCL18 基因编码。也称为 MIP-4，它与人类 MIP-1α 具有 61% 的序列同一性。 CCL18主要在肺表达，一些淋巴组织如淋巴结低水平表达CCL18。它对活化 (CD3+) T 细胞和非活化 (CD14-) 淋巴细胞具有趋化作用，但对单核细胞或粒细胞没有趋化作用。参与 B 细胞迁移至淋巴结中的 B 细胞滤泡。 CCL18 在体液和细胞介导的免疫反应中发挥作用。
Reference
[1]. Hieshima K, Imai T, Baba M, et al. 1997. J Immunol, 159: 1140-9.
[2]. Chenivesse C, Chang Y, Azzaoui I, et al. 2012. J Immunol, 189: 128-37.
[3]. Azzaoui I, Yahia SA, Chang Y, et al. 2011. Blood, 118: 3549-58.
[4]. Adema GJ, Hartgers F, Verstraten R, et al. 1997. Nature, 387: 713-7.
| Accession # | P1219 |
| Alternate Names | AMAC-1, PARC, DC-CK1 |
| Source | Escherichia coli. |
| Protein sequence | AQVGTNKELC CLVYTSWQIP QKFIVDYSET SPQCPKPGVI LLTKRGRQIC ADPNKKWVQK YISDLKLNA |
| M.Wt | 7.9 kDa |
| Appearance | Solution protein |
| Stability & Storage | Avoid repeated freeze-thaw cycles It is recommended that hat the protein be aliquoted for optimal storage - 12 months from date of receipt, -20 to -7°C as supplied |
| Concentration | 0.1-1.0 mg/mL |
| Formulation | Dissolved in Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This solution can be diluted into other aqueous buffers. |
| Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 1.0-10 ng/ml. |
| Shipping Condition | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
质量控制和DataSheet
- 批次:
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纯度 > 95%, determined by SDS-PAGE.
- Datasheet
Endotoxin: <1 EU per 1 ug of the protein by the LAL method.