Recombinant Human Macrophage Inflammatory Protein-5/CCL15
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
人CCL15属于CC趋化因子家族，与人CCL14有35%的氨基酸同源性。CCL15在心脏、骨骼肌和肾上腺中含量最高，在肝脏、小肠、结肠和肺中某些白细胞和巨噬细胞中低表达。它对中性粒细胞、单核细胞和淋巴细胞具有趋化作用，并通过与细胞表面趋化因子受体CCR1和CCR3结合而发挥作用。CCL15有几条裂解链。它们都是比CCL15更强的化学引诱剂。
Reference
1. Youn BS, Zhang SM, Broxmeyer HE, et al. 1998. Blood. 91:3118-26
2. Berahovich RD, Miao Z, Wang Y, et al. 2005. J Immunol. 174:7341-51
3. Pardigol A, Forssmann U, Zucht HD, et al. 1998. Proc Natl Acad Sci U S A. 95:6308-13
4. Youn BS, Zhang SM, Lee EK, et al. 1997. J Immunol. 159:5201-5
5. Coulin F, Power CA, Alouani S, et al. 1997. Eur J Biochem. 248:507-15.
| Gene ID | 6359 |
| Accession # | Q16663 |
| Alternate Names | C-C Motif Chemokine 15, LKN-1, Mrp-2b, NCC-3, Small-inducible Cytokine A15 |
| Source | Escherichia coli. |
| M.Wt | Approximately 10.2 kDa, a single non-glycosylated polypeptide chain containing 92 amino acids. |
| AA Sequence | QFINDAETEL MMSKLPLENP VVLNSFHFAA DCCTSYISQS IPCSLMKSYF ETSSECSKPG VIFLTKKGRQ VCAKPSGPGV QDCMKKLKPY SI |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/ml. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
质量控制和DataSheet
- 批次:
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纯度 > 97 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rHuMIP-5/CCL15 as determined by LAL method.