Recombinant Human Interleukin-1 Receptor Antagonist Protein
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
白介素1受体拮抗剂(IL-1RA)是IL-1家族的成员。IL1RA由免疫细胞、上皮细胞和脂肪细胞等多种细胞分泌，是IL1β促炎作用的天然抑制剂。该蛋白抑制白介素1α(IL1A)和白介素1β(IL1B)的活性，并调节各种与白介素1相关的免疫和炎症反应。IL-4和IL-13可增强IL-1-β对细胞内和可溶性IL-1-RA产生的刺激作用。IL1RA在不同细胞类型中的调节表达已被证明受到细胞因子的影响。在滑膜成纤维细胞中，IL-1、TNF-α或PDGF显著促进IL-1RA的合成。该蛋白与IL-1β的氨基酸同源性为26%，与IL-1α的同源性为19%。
Reference
1. Dinarello, C.A. 1994. FASEB J, 8: 1314-25
2. Perrier, S., F. Darakhshan, and E. Hajduch. 2006. FEBS Lett, 580: 6289-94
3. Dinarello, C.A. 1994. FASEB J, 8: 1314-25.
| Gene ID | 3557 |
| Accession # | P18510 |
| Alternate Names | IL-1RN, IRAP |
| Source | Escherichia coli. |
| M.Wt | Approximately 17.1 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids. |
| AA Sequence | RPSGRKSSKM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNV NLEEKIDVVP IEPHALFLGI HGGKMCLSCV KSGDETRLQL EAVNITDLSE NRKQDKRFAF IRSDSGPTTS FESAACPGWF LCTAMEADQP VSLTNMPDEG VMVTKFYFQE DE |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1αdependent proliferation of murine D10S cells is less than 40 ng/ml, corresponding to a specific activity of > 2.5 ×104 IU/mg in the presence of 50 pg/ml rHuIL-1α |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
质量控制和DataSheet
- 批次:
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纯度 > 96 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rHuIL-1RA as determined by LAL method.