Recombinant Human Epithelial Neutrophil Activating Peptide-78, 8-78 a.a./CXCL5
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
CXCL5 是 CXC 趋化因子家族的成员，也称为上皮来源的中性粒细胞活化肽 78 （ENA-78）。它是在用炎性细胞因子白细胞介素-1 或肿瘤坏死因子-α 刺激细胞后产生的。在体外，ENA-78 （8-78） 和 ENA-78 （9-78） 对中性粒细胞的趋化活性高出三倍。它们由外周血单核细胞分泌后蛋白水解裂解产生。重组人 CXCL5 （8-78 a.a.） 含有 71 个氨基酸，是一条非糖基化的多肽链。人 CXCL5 与小鼠和大鼠 CXCL5 具有 57% 的氨基酸序列同一性。
Reference
1. Chang MS, McNinch J, Basu R, et al. 1994. J Biol Chem. 269:25277-82
2. O'Donovan N, Galvin M, Morgan JG. 1999. Cytogenet Cell Genet. 84:39-42
3. Persson T, Monsef N, Andersson P, et al. 2003. Clin Exp Allergy. 33:531-7
4. Wuyts A, Govaerts C, Struyf S, et al. 1999. Eur J Biochem. 260:421-9.
| Gene ID | 6374 |
| Accession # | P42830 |
| Alternate Names | CXCL5, Small-inducible cytokine B5 |
| Source | Escherichia coli. |
| M.Wt | Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 71 amino acids. |
| AA Sequence | LRELRCVCLQ TTQGVHPKMI SNLQVFAIGP QCSKVEVVAS LKNGKEICLD PEAPFLKKVI QKILDGGNKE N |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 5.0-10.0 ng/ml. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
质量控制和DataSheet
- 批次:
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纯度 > 95 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rHuENA-78, 8 - 78 a.a./CXCL5 as determined by LAL method.