Catalog No. B7777

MTT

Name: MTT,噻唑蓝,Thiazolyl Blue,298-93-1
Brand: APExBIO
SKU: B7777
Price: 345 CNY
Availability: InStock

（别名：Thiazolyl Blue, 噻唑蓝）

用于检测体外细胞增殖的试剂

规格	价格	货期	数量
500mg	￥345.00	现货

CAS号：298-93-1纯度：99.50%

仅用于科研，不用于诊疗。未经明确授权不得转售。

021-55669583sales@apexbio.cnAPExBIO

特色产品

Annexin V-FITC/PI Apoptosis Kit

用于双染检测细胞凋亡和坏死

- 在10-20分钟内完成染色
- 适用于流式细胞仪和荧光显微镜检测
- 准确区分活细胞/凋亡细胞/坏死细胞

Phos binding reagent (Phosbind) acrylamide

分离和检测磷酸化/非磷酸化蛋白

- 新型的磷酸盐结合标签和功能分子
- 用于免疫印迹和质谱分析等后续操作
- 适用于30 KDa-130 KDa大小的蛋白

Cell Counting Kit-8 (CCK-8)

用于细胞增殖和细胞毒性试验

- 比MTT，MTS或WST-1更敏感
- 对细胞无毒性
- 步骤更简单，无需有机溶剂

Cy5 TSA Fluorescence System Kit

Cy5荧光标记的酪胺信号放大系统

- 检测ICC/IHC/ISH中的低丰度靶点
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率

EZ Cap™ Cy5 Firefly Luciferase mRNA (5-moUTP)

用于观察mRNA运送，定位，翻译

- 具有Cap1结构的荧光素酶mRNA
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰

HotStart™ 2X Green qPCR Master Mix

用于荧光定量目标DNA或cDNA

- 通过热启动机制优化扩增特异性
- 优异的扩增效率、可重复性和稳定性
- 卓越的灵敏度和准确性，操作简单

HyperScribe™ T7 High Yield Cy5 RNA Labeling Kit Plus

高效的Cy5修饰RNA转录试剂盒

- 适合原位/Northernblot杂交实验
- 优化的缓冲液和T7 RNA聚合酶混合物
- 20 μL反应体系可获得更高的RNA产量

产品描述产品性质产品应用生物相关数据质量控制操作说明产品引用文献摩尔浓度计算器

产品描述

IC50：N/A。

MTT（3-（4,5-二甲基-2-噻唑基）-2,5-二苯基-2H-溴化四唑）是一种用于检测体外细胞增殖的试剂。

在细胞生物学中，四唑盐已被广泛用于检测从微生物细胞到哺乳动物细胞的代谢活性。哺乳动物细胞的组分研究表明，NADH（还原型吡啶核苷酸辅因子）能使大多数MTT还原，全细胞研究也进一步证明上述结论。

体外：MTT还原不仅与线粒体相关，同时也与细胞质以及非线粒体膜（包括核内体膜、溶酶体膜和质膜）相关。MTT所带的净正电荷是通过质膜电位参与其细胞摄取过程的主要因子。然而，第2代四唑染料（XTT、WST-1以及MTS）形成水溶性formazans，需要用于还原反应的中间电子受体。由于第2代四唑染料携带一个净负电荷，因而其细胞渗透性极差[1]。

体内：目前，MTT仅用于细胞体外增殖测定，暂无体内研究。

参考文献：
[1] Berridge MV, Herst PM, Tan AS. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev. 2005;11:127-52.

产品性质

物理外观	黄色固体
CAS号	298-93-1
分子式	C₁₈H₁₆N₅SBr
分子量	414.32
小分子别名	Thiazolyl Blue
化学名称	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazol-3-ium bromide
溶解度	≥41.4 mg/mL in DMSO; ≥18.63 mg/mL in EtOH; ≥2.5 mg/mL in H2O with ultrasonic
SMILES	Cc1c(C)[s]c(-[n+]2nc(-c3ccccc3)n[n]2-c2ccccc2)n1.[Br-]
存储条件	-20°C
运输条件	蓝冰
注意事项	如需配成储存母液，请分装保存，避免反复冻融。

产品应用 (实验数据来自文献，APExBIO并未验证，仅供参考)

IC50和靶点

生物活性描述

噻唑蓝（MTT）是一种细胞渗透性和带正电荷的比色剂，广泛用于检测细胞中的还原代谢，以及测量细胞增殖、细胞毒性和细胞凋亡。噻唑蓝（MTT）在活细胞中会从黄色还原成紫色的甲臢。

生物相关数据

质量控制

请选择批次查看:

纯度 = 99.50%

COA (Certificate Of Analysis)HPLC NMR (Nuclear Magnetic Resonance) (Retest)MSDS (Material Safety Data Sheet)

操作说明

Datasheet

APExBIO 顾客使用本产品发表的 37 篇科研文献

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6. Weinan Li, Yuhan Fu, et al. "Construction and in vitro evaluation of pH-sensitive nanoparticles to reverse drug resistance of breast cancer stem cells." Discov Oncol. 2024 Jan 29;15:21. PMID: 38285118

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14. Liang Li, Gao Li, et al. "Astragaloside IV enhances the sensibility of lung adenocarcinoma cells to bevacizumab by inhibiting autophagy." Drug Dev Res. 2022 Apr;83(2):461-469. PMID: 34499759

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16. Jianyan Qi, Min Gong, et al. "Evaluation of the antibacterial effect of tea tree oil on Enterococcus faecalis and biofilm in vitro." J Ethnopharmacol. 2021 Dec 5;281:114566. PMID: 34450163

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摩尔浓度计算器

质量

= 浓度

x 体积

x 分子量

*在配置溶液时，请务必参考产品标签上、MSDS / COA（可在Apexbio的产品页面获得）批次特异的分子量使用本工具。

MTT