MTT
噻唑蓝
Catalog No.
B7777
用于检测体外细胞增殖的试剂
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MTT（3-（4,5-二甲基-2-噻唑基）-2,5-二苯基-2H-溴化四唑）是一种用于检测体外细胞增殖的试剂。
在细胞生物学中，四唑盐已被广泛用于检测从微生物细胞到哺乳动物细胞的代谢活性。哺乳动物细胞的组分研究表明，NADH（还原型吡啶核苷酸辅因子）能使大多数MTT还原，全细胞研究也进一步证明上述结论。
体外：MTT还原不仅与线粒体相关，同时也与细胞质以及非线粒体膜（包括核内体膜、溶酶体膜和质膜）相关。MTT所带的净正电荷是通过质膜电位参与其细胞摄取过程的主要因子。然而，第2代四唑染料（XTT、WST-1以及MTS）形成水溶性formazans，需要用于还原反应的中间电子受体。由于第2代四唑染料携带一个净负电荷，因而其细胞渗透性极差[1]。
体内：目前，MTT仅用于细胞体外增殖测定，暂无体内研究。
参考文献：
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| Storage | Store at -20°C |
| M.Wt | 414.32 |
| Cas No. | 298-93-1 |
| Formula | C18H16N5SBr |
| Solubility | ≥41.4 mg/mL in DMSO; ≥18.63 mg/mL in EtOH; ≥2.5 mg/mL in H2O with ultrasonic |
| Chemical Name | 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazol-3-ium bromide |
| SDF | Download SDF |
| Canonical SMILES | CC1=C(C)N=C([N+]2=NC(C3=CC=CC=C3)=NN2C4=CC=CC=C4)S1.[Br-] |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
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