Longdaysin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Longdaysin是一种酪蛋白激酶抑制剂，可以抑制Wnt/β-catenin信号通路抑制剂，抑制CKIδ，CKIα，ERK2和CDK7的IC50值分别为8.8 μM，5.6 μM，52 μM和29 μM[1]。在乳腺癌Hs578T和MDA-MB-231细胞中，微摩尔浓度的longdaysin减弱了LRP6和DVL2的磷酸化，并降低了活性β-catenin和总β-catenin的表达，导致 Wnt 靶基因 Axin2、DKK1、LEF1和Survivin下调[2]。 在MDA-MB-231乳腺癌异种移植物中，longdaysin 治疗抑制了肿瘤生长并抑制了Wnt/β-catenin 信号传导[2]。
参考文献：
[1]. Hirota T, et al. High-throughput chemical screen identifies a novel potent modulator of cellular circadian rhythms and reveals CKIα as a clock regulatory kinase. PLoS Biol. 2010 Dec 14;8(12):e1000559. PMID: 21179498
[2]. Xiong Y, et al. Longdaysin inhibits Wnt/β-catenin signaling and exhibits antitumor activity against breast cancer. Onco Targets Ther. 2019 Feb 5;12:993-1005. PMID: 30787621
| Storage | Store at -20°C |
| M.Wt | 335.33 |
| Cas No. | 1353867-91-0 |
| Formula | C16H16F3N5 |
| Solubility | ≥33.3 mg/mL in DMSO |
| Chemical Name | 9-isopropyl-N-(3-(trifluoromethyl)benzyl)-9H-purin-6-amine |
| SDF | Download SDF |
| Canonical SMILES | FC(F)(F)C1=CC=CC(CNC2=NC=NC3=C2N=CN3C(C)C)=C1 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
