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L-Leucine 4-methoxy-β-naphthylamide (hydrochloride)

 
Catalog No.
C3708
氨基肽酶M和亮氨酸氨基肽酶的底物
组合的产品项目
规格价格库存 数量
100mg
¥ 1,300.00
Ship with 10-15 days
250mg
¥ 3,033.00
Ship with 10-15 days
1g
¥ 7,800.00
Ship with 10-15 days

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A

背景

L-Leucine 4-methoxy-β-naphthylamide is a substrate for aminopeptidase M and leucine aminopeptidase.

Aminopeptidases are enzymes catalyzing the cleavage of amino acids from the amino terminus (N-terminus) of proteins or peptides. Aminopeptidases are distributed widely throughout the animal and plant kingdoms and are present in various subcellular organelles, in cytosol, as well as membrane components.

In vitro: L-Leucine 4-methoxy-β-naphthylamide was identified as a cell-permeable substrate for aminopeptidase M and leucine aminopeptidase that was developed for intracellular analysis of protease activities [1]. In a previous method-developing study, peptide derivatives of 4-methoxy-β-naphthylamine including L-leucine 4-methoxy-β-naphthylamide were incubated in microtiter plates together with nitrosalicylaldehyde. Selectivity was achieved by running parallel assays containing inhibitors partially selective for each of peptide derivatives. This method was successfully validated by measurements in cells isolated from cathepsin B-/--, K-/--, and L-/-- mice [2].

In vivo: Up to now, there is no animal in vivo data reported.

Clinical trial: So far, no clinical study has been conducted.

References:
[1] Monis, B. ,Wasserkrug, H. and Seligman, A.M. Comparison of fixatives and substrates for aminopeptidase. Journal of Histochemistry and Cytochemistry 13(6), 503-509 (1965).
[2] Rüttger, A. ,Mollenhauer, J.,Lser, R., et al. Microplate assay for quantitative determination of cathepsin activities in viable cells using derivatives of 4-methoxy-β-naphthylamide. Biotechniques 41(4), 469-473 (2006).

化学属性

Physical AppearanceA crystalline solid
StorageStore at -20°C
M.Wt322.8
Cas No.4467-68-9
FormulaC17H22N2O2·HCl
SynonymsLeu-MNA
Solubility≤25mg/ml in ethanol;10mg/ml in DMSO;20mg/ml in dimethyl formamide
Chemical Name(S)-2-amino-N-(4-methoxy-2-naphthalenyl)-4-methyl-pentanamide, monohydrochloride
SDFDownload SDF
Canonical SMILESCOC1=CC(NC([C@H](CC(C)C)N)=O)=CC2=CC=CC=C21.Cl
运输条件 蓝冰运输或根据您的需求运输。
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质量控制

质量控制和MSDS

批次:

化学结构

L-Leucine 4-methoxy-β-naphthylamide (hydrochloride)