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IL-7, human recombinant

 
Catalog No.
P1024
IL-7,淋巴细胞生长因子,在大肠杆菌中表达,种间反应性。
组合的产品项目
规格价格库存 数量
10ug
¥ 2,216.00
Ship with 5-10 days
100ug
¥ 12,686.00
Ship with 5-10 days
500ug
¥ 28,463.00
Ship with 5-10 days

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A

背景

Interleukin-7 (IL-7) is encoded by the IL7 gene and secreted by stromal cells in the red marrow and thymus. It binds to the IL-7 receptor, a heterodimer consisting of IL-7 receptor alpha and IL-2 receptor gamma chain. IL-7 stimulates the differentiation of hematopoietic stem cells into lymphoid progenitor cells and also stimulates proliferation of B cells, T cells and NK cells. Murine IL-7 has approximately 65 % amino acid sequence identity with human IL-7 and both proteins exhibit cross-speciesactivity. IL-7 as an immunotherapy agent has been examinedin many human clinical trials for various malignancies and during HIV infection.

Reference:

1. Goodwin RG, Lupton S, Schmierer A, et al. 1989. Proc Natl Acad Sci U S A. 86:302-6

2. Sutherland GR, Baker E, Fernandez KE, et al. 1989. Hum Genet. 82:371-2

3. Lupton SD, Gimpel S, Jerzy R, et al. 1990. J Immunol. 144:3592-601.

4. Noguchi M, Nakamura Y, Russell SM, et al. 1993. Science. 262:1877-80

5. Fry TJ, Mackall CL. 2002. Blood. 99:3892-904

6. Fry TJ, Mackall CL. 2002. J Hematother Stem Cell Res. 11:803-7.

Description

Gene ID

3574

Accession #

P13232

Alternate Names

LP-1, pre-B cell factor

Source

Escherichia coli.

M.Wt

Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.

AA Sequence

DCDIEGKDGK QYESVLMVSI DQLLDSMKEI GSNCLNNEFN FFKRHICDAN KEGMFLFRAA RKLRQFLKMN STGDFDLHLL KVSEGTTILL NCTGQVKGRK PAALGEAQPT KSLEENKSLK EQKKLNDLCF LKRLLQEIKT CWNKILMGTK EH

Purity

> 97% by SDS-PAGE and HPLC analyses.

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Formulation

 

Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Biological Activity

Fully biologically active when compared to standard. rHuIL-7 stimulates proliferation of PHA-activated human peripheral blood mononuclear cell (PBMC). The specific activity of Recombinant Human IL-7 is ≥1.0×108 IU/mg, which is calibrated against human IL-7 WHO Standard (NIBSC code: 90/530).

Shipping Condition

Gel pack.

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans.

 

质量控制

Quality Control & DataSheet

View current batch:
    Purity: > 97 % by SDS-PAGE and HPLC analyses.
    Endotoxin Level: Less than 1 EU/µg of rHuIL-7 as determined by LAL method.
  • Datasheet

相关生物数据

IL-7, human recombinant