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IL-15, human recombinant protein

 
Catalog No.
P1029
IL-15,在大肠杆菌中表达的免疫调节细胞因子,对鼠细胞有活性。
组合的产品项目
规格价格库存 数量
10ug
¥ 2,216.00
Ship with 5-10 days
100ug
¥ 9,512.00
Ship with 5-10 days
500ug
¥ 21,342.00
Ship with 5-10 days

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背景

Human Interleukin-15 (IL-15) is expressed by the IL15 gene located on the chromosome 4. It shares approximately 97 % and 73 % sequence identity with simian and murine IL-15, respectively. Both human and simian IL-15 are active on murine cells. IL-15 is secreted by mononuclear phagocytes (and some other cells), especially macrophages following infection by virus. It possesses a variety of biological functions, including stimulating and maintaining of cellular immune responses, especially regulating T and natural killer (NK) cell activation and proliferation. In additionally, it shares many biological properties with IL-2, including T, B and NK cell-stimulatory activities. IL-15 signals through a complex composed of IL-2/IL-15 receptor beta chain. Although IL-15 lacks sequence homology with IL-2, it has recently been shown that both the beta and gamma chains of the IL-2 receptor are utilized for IL-15 binding and signaling. In addition, an IL-15 specific binding protein has also been cloned from a mouse T cell clone.

Reference:

1. Anderson DM, Johnson L, Glaccum MB, et al. 1995. Genomics, 25: 701-6

2. Krause H, Jandrig B, Wernicke C, et al. 1996. Cytokine, 8: 667-74

3. Chirifu M, Hayashi C, Nakamura T, et al. 2007. Nat Immunol, 8: 1001-7

4. Grabstein KH, Eisenman J, Shanebeck K, et al. 1994. Science, 264: 965-8

5. Giri JG, Ahdieh M, Eisenman J, et al. 1994. EMBO J, 13: 2822-30

6. Arena A, Merendino RA, Bonina L, et al. 2000. New Microbiol, 23: 105-12.

Description

Gene ID

3600

Accession #

P40933

Alternate Names

 

Source

Escherichia coli.

M.Wt

Approximately 12.9 kDa, a single non-glycosylated polypeptide chain containing 114 amino acids.

AA Sequence

NWVNVISDLK KIEDLIQSMH IDATLYTESD VHPSCKVTAM KCFLLELQVI SLESGDASIH DTVENLIILA NNSLSSNGNV TESGCKECEE LEEKNIKEFL QSFVHIVQMF INTS

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Formulation

 

Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Biological Activity

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 0.300-2.60 ng/mL. The specific activity of recombinant human IL-15 is ≥1.50 ×108units/mg, which is calibrated against the human IL-15 reference standard (NIBSC code: 95/554).

Shipping Condition

Gel pack.

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans.

 

质量控制

Quality Control & DataSheet

View current batch:
    Purity: > 97 % by SDS-PAGE and HPLC analyses.
    Endotoxin Level: Less than 1 EU/µg of rHuIL-15 as determined by LAL method.
  • Datasheet

相关生物数据

IL-15, human recombinant protein