EZ Cap™ Cy5 EGFP mRNA (5-moUTP)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
EZ Cap™Cy5 EGFP mRNA(5-moUTP)最初是从水母(Aequorea victoria)分离的,一旦进入的细胞,产品将表达增强型绿色荧光蛋白(EGFP)。您可以在509 nm处观察到绿色荧光。 EGFP通常用作基因调控和功能研究的报告基因。它适用于mRNA运送,翻译效率,细胞活力和体内成像等测定。
EZ Cap™ Cy5 EGFP mRNA(5-moUTP)以1mg / ml的浓度提供。它使用EZ Cap™ Reagent AG(货号:B8176)以共转录的方式加帽,以较高效率获得具有Cap 1结构的mRNA。 相比于Cap 0结构(ARCA和mCap加帽),带有Cap 1结构的mRNA对于哺乳动物系统更适合,并且具有更高的转录效率。添加5-moUTP(5-Methoxy-UTP)和poly(A)尾可抑制RNA介导的先天免疫激活,并在体外和体内增强mRNA的稳定性和寿命。 Poly(A)尾在提高翻译起始效率方面也起着重要作用。 Cy5是合成的红色荧光染料,其最大激发和发射波长分别为650nm和670nm。当转录时,Cy5-UTP和5-moUTP以1:3的比例使用。以该比例使用可以使mRNA易于可视化并且仍然可以在细胞培养物中翻译。一般,翻译效率和Cy5-UTP替代之间存在反相关。
产品的所有修饰都旨在模拟完全加工成熟的mRNA。 EZ Cap™ Cy5 EGFP mRNA(5-moUTP)是观察mRNA运送,定位,翻译和其他行为的理想产品。
- 1. Zhihui Dong, Zhuoshan Huang, et al. "Nanoparticles (NPs)-mediated systemic mRNA delivery to reverse trastuzumab resistance for effective breast cancer therapy." Acta Pharmaceutica Sinica B.
- 2. Valentina Andretto, Mathieu Repellin, et al. "Hybrid core-shell particles for mRNA systemic delivery ." J Control Release. PMID: 36442614
- 3. Chen Q, Gao M, et al. "Biodegradable nanoparticles decorated with different carbohydrates for efficient macrophage-targeted gene therapy." J Control Release. 2020 Apr 22;323:179-190. PMID: 32334322
mRNA Length | 996 nucleotides | ||
Concentration | 1 mg/mL | ||
Buffer | 1 mM Sodium Citrate, pH 6.4 | Storage | -40°C or below |
General tips | 请将其于冰上溶解,并小心防止RNase污染降解。尽可能避免反复冻融。 不要涡旋震荡。首次使用时,将其轻柔离心并分成几份,可供单独使用。使用不含RNase的试剂和耗材,使用适当的无RNase技术。直至与转染试剂混合,才可加入含有血清的培养基中。 | ||
Shipping Condition | 试用装:干冰运输。 |