EZ Cap™ Human P16 mRNA (5-moUTP)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
P16基因是一种细胞周期中的基本基因,直接参与细胞周期的调控信号通路,其在肿瘤形成和发展中起到重要作用。它可以抑制细胞先天或后天的致癌突变,通过阻碍细胞周期进程和诱导细胞老化来抑制肿瘤细胞的生长和扩散。失活或缺失的p16基因与多种肿瘤的发生密切相关,包括黑色素瘤、胰腺癌和宫颈癌等。
EZ Cap™ Human P16 mRNA (5-moUTP) 浓度约为1 mg/mL,表达人源P16蛋白。通过共转录加帽生成Cap 1结构。Cap 1结构更适合哺乳动物系统,并且比Cap 0结构(ARCA和mCap)具有更高的转录效率。添加5-moUTP和poly (A) tail抑制RNA介导的先天免疫激活,并增加mRNA在体外和体内的稳定性和寿命。poly (A) tail 在提高翻译启动效率方面也起着重要作用。
mRNA Length |
726 nucleotides |
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Concentration |
~1 mg/mL |
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Buffer |
1 mM Sodium Citrate, pH 6.4 |
Storage |
-40°C or below |
General tips |
请将其于冰上溶解,并小心防止RNase污染降解。尽可能避免反复冻融,不要涡旋震荡。首次使用时,将其轻柔离心并分成几份,可供单独使用。使用不含RNase的试剂和耗材,使用适当的无RNase技术。直至与转染试剂混合,才可加入含有血清的培养基中。 |
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Shipping Condition |
干冰运输 |