EZ Cap™ Human PTEN mRNA
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
PTEN (deletion of phosphatase and tensin homologues on chromosome 10) is a tumor suppressor gene that resists the action of PI3K, which is a key enzyme for anti-apoptotic, pro-tumorigenic Akt activation.
EZ Cap™ Human PTEN mRNA is provided at a concentration of 1mg/ml. It is co-transcriptional capped which generates a cap 1 structure with high efficiency. Cap 1 structure is more ideal for mammalian systems and possesses higher transcription efficiency than Cap 0 structure (ARCA and mCap). The addition of poly (A) tail increases the stability and lifetime of the mRNA in vitro and in vivo. Poly (A) tail also plays an important role in enhancing the efficiency of translation initiation.
| mRNA Length | 1467 nucleotides |
| Concentration | 1 mg/mL |
| Buffer | 1 mM Sodium Citrate, pH 6.4 |
| Storage | -40°C or below |
| General tips | 请将其于冰上溶解,并小心防止RNase污染降解。尽可能避免反复冻融，不要涡旋震荡。首次使用时，将其轻柔离心并分成几份，可供单独使用。使用不含RNase的试剂和耗材，使用适当的无RNase技术。直至与转染试剂混合，才可加入含有血清的培养基中。 |
| Shipping Condition | 干冰运输 |