ETP-46464
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
ETP-46464是高效选择性ATR抑制剂(IC50 = 25 nM).
ATR (ATM- and Rad3-related)是PIKK(磷脂酰肌醇 3-激酶-样-激酶)家族中的一员,调节DNA损伤应答通路.ATR是DNA损伤感受器,受遗传毒性应力(如电离辐射\紫外照射和DNA复制失控)而活化,并磷酸化其下游底物(如p53\BRCA1和CHEK1).
ETP-46464废除G2/M检查点,引起电离辐射后的细胞中出现微核或完全碎片核.Hydroxyurea和ETP-46464同时处理的细胞表现出ATM和Chk2磷酸化的提高.U2OS细胞中ETP-46464促进复制叉停滞的破损[1].
参考文献：
1. Toledo LI, Murga M, Zur R et al. A cell-based screen identifies ATR inhibitors with synthetic lethal properties for cancer-associated mutations. Nat Struct Mol Biol. 2011 Jun;18(6):721-7.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 470.52 |
| Cas No. | 1345675-02-6 |
| Formula | C30H22N4O2 |
| Solubility | ≥16.13 mg/mL in DMSO |
| Chemical Name | 2-methyl-2-[4-(2-oxo-9-quinolin-3-yl-4H-[1,3]oxazino[5,4-c]quinolin-1-yl)phenyl]propanenitrile |
| SDF | Download SDF |
| Canonical SMILES | CC(C)(C#N)C1=CC=C(C=C1)N2C3=C4C=C(C=CC4=NC=C3COC2=O)C5=CC6=CC=CC=C6N=C5 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Description | ETP-46464是高效选择性ATR抑制剂,IC50值为25 nM. | |||||
| 靶点 | mTOR | ATR | DNA-PK | PI3Kα | ATM | |
| IC50 | 0.6 nM | 14 nM | 36 nM | 170 nM | 545 nM | |
质量控制和MSDS
- 批次:
化学结构
