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(CXCL12), human recombinant protein

 
Catalog No.
P1042
SDF-1α(CXCL12),趋化因子,在大肠杆菌中表达,种间反应性。
组合的产品项目
规格价格库存 数量
10ug
¥ 2,216.00
Ship with 5-10 days
100ug
¥ 7,317.00
Ship with 5-10 days
500ug
¥ 16,419.00
Ship with 5-10 days

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背景

CXCL12 also known as SDF-1 is belonging to the CXC chemokine family. It is encoded by the CXCL12 gene. In recently study, Human CXCL12 is expressed as six isoforms that differ only in the C-terminal tail. And all SDF-1 isoforms undergo proteolytic processing of the first two N-terminal amino acids. Contrast to the canonical sequence SDF-1β, SDF-1α is shorter by four amino acids at the C-terminal tail. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. CXCL12 is a very important factor in carcinogenesis and the neovascularisation linked to tumor progression. 

Reference:

1. Shirozu M, Nakano T, Inazawa J, et al. 1995. Genomics. 28:495-500.

2. Yu L, Cecil J, Peng SB, et al. 2006. Gene. 374:174-9

3. De La Luz Sierra M, Yang F, Narazaki M, et al. 2004. Blood. 103:2452-9

4. Charnaux N, Brule S, Hamon M, et al. 2005. FEBS J. 272:1937-51

5. Bleul CC, Fuhlbrigge RC, Casasnovas JM, et al. 1996. J Exp Med. 184:1101-9

6. Ara T, Nakamura Y, Egawa T, et al. 2003. Proc Natl Acad Sci U S A. 100:5319-23

7. Askari AT, Unzek S, Popovic ZB, et al. 2003. Lancet. 362:697-703

8. Ma Q, Jones D, Borghesani PR, et al. 1998. Proc Natl Acad Sci U S A. 95:9448-53

9. Kryczek I, Wei S, Keller E, et al. 2007. Am J Physiol Cell Physiol. 292:C987-95.

Description

Gene ID

6387

Accession #

P48061

Alternate Names

SDF-1 alpha, hSDF-1 alpha, IRH, hIRH, PBSF

Source

Escherichia coli.

M.Wt

Approximately 8.0 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids.

AA Sequence

KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Formulation

 

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB pH 7.0, 130 mM NaCl.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using PHA and rHuIL-2 activated human peripheral blood T-lymphocytes is in a concentration range of 20-80 ng/ml.

Shipping Condition

Gel pack.

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans.

 

质量控制

质量控制和DataSheet

批次:
    纯度 > 97 % by SDS-PAGE and HPLC analyses.
    Endotoxin: Less than 1 EU/μg of rHuSDF-1α/CXCL12α as determined by LAL method.
  • Datasheet

相关生物数据

FGF-21

相关生物数据

FGF-21