Biotinylated Recombinant Human Macrophage Migration Inhibitory Factor, His, Avi
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
迁移抑制因子(MIF)是一种没有可切割信号序列的分泌型蛋白质，通过一种特殊的、非经典的途径分泌。它由巨噬细胞在细菌脂多糖或结核分枝杆菌抗原刺激下分泌。MIF由两个α-螺旋和六个β-链组成，其中四个形成β-Sheet。剩下的两条β链与其他mif分子相互作用，形成一个三聚体。结构-功能研究表明，MIF具有双功能，具有分离的拓扑结构。N-末端和C-末端是酶活性的中介体(理论上)。苯丙酮酸互变异构酶活性(烯醇-酮基)已被证明，并依赖于第1位的Pro。也被认为含有硫醇蛋白氧化还原酶活性。MIF的促炎细胞因子活性集中在49-65(A.A.)。在成纤维细胞上，MIF诱导IL-1、IL-8和基质金属蛋白酶的表达，在巨噬细胞上，MIF刺激NO的产生和肿瘤坏死因子-α的释放，继而激活干扰素-γ。MIF显然通过CD74和CD44发挥作用，可能是以某种形式的三聚体相互作用。人MIF在小鼠细胞上是活性的。人的MIF分别为90%、94%、95%和90%。分别与小鼠、牛、猪和大鼠的MIF相同。
| Source | Escherichia coli. |
| M.Wt | Approximately 15.4 kDa, a single non-glycosylated polypeptide chain containing 136 amino acids, with 6 × His and Avi tag at the C-terminus. |
| AA Sequence | MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFAHHHHH HGLNDIFEAQ KIEWHE |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0, 5 % Trehalose, 0.02 % Tween-20. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Fully biologically active when compared to standard. The specific activity is determined by binding rhCD74 in a functional ELISA. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
质量控制和DataSheet
- 批次:
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纯度 > 97 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 0.1 EU/μg of rHuMIF, His, Avi, Biotinylated as determined by LAL method.