Recombinant Human USP2 Protein
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
泛素特异性蛋白酶（ubiquitin-specific proteases，USPs）是去泛素化酶（deubiquitylating enzyme，DUBs）家族最大的亚族，该族成员通过催化移除靶蛋白的泛素修饰，从而抑制靶蛋白的泛素-蛋白酶体途径降解或者影响靶蛋白的内吞、转运以及活性，是肿瘤干扰治疗的重要潜在靶标。
去泛素化蛋白酶（USP2），也称为UBP41和USP9，是一种细胞质蛋白，属于去泛素化酶肽酶C19家族。USP2广泛表达并作用于去泛素化选定的目标底物，涉及肿瘤、生物钟、代谢、炎症等过程。到目前为止，已知至少有三种USP2亚型，即USP2a（也称USP2-69），USP-41和USP2-45。其所有亚型都包含相同的催化核心，但在其N端和C端结构域内表现出变化。
USP2a是长度为605个氨基酸，预测分子量为68 kDa的规范亚型。人类USP2a与小鼠和大鼠直系同源物共享90%的氨基酸序列同一性。USP-41和USP-45的长度分别为362和396 个氨基酸，预测分子量为41和45 kDa。USP-41和USP2a已被证明是细胞凋亡和细胞增殖的关键调节因子。它们对多泛素化的靶蛋白（如FAS、MDM2和细胞周期蛋白D1）进行去泛素化，以促进或阻断细胞凋亡，具体取决于具体情况。USP-45的表达似乎受昼夜节律和营养线索的调节，被认为可调节肝糖异生和葡萄糖代谢。重组人去泛素化蛋白酶2氨基酸序列为第259到第605位的催化结构域。
|
Name |
Recombinant Human USP2 Protein |
|
Species |
Human |
|
Source |
E. coli-derived human USP2 protein |
|
Storage Buffer: |
50 mM HEPES pH 7.5, 100 mM NaCl, and 1 mM DTT |
|
Purity |
>95% SDS-PAGE |
|
Amino acids |
259 to 605 |
|
MW |
~41 kDa |
|
Tag |
His tag C-Terminus |
|
Biological Activity |
Recombinant Human USP2 Catalytic Domain is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP2 Catalytic Domain concentration of 1-5 nM. |
|
Amino Acid Sequence |
MNSKSAQGLAGLRNLGNTCFMNSILQCLSNTRELRDYCLQRLYMRDLHHGSNAHTALVEEFAKLIQTIWTSSPNDVVSPSEFKTQIQRYAPRFVGYNQQDAQEFLRFLLDGLHNEVNRVTLRPKSNPENLDHLPDDEKGRQMWRKYLEREDSRIGDLFVGQLKSSLTCTDCGYCSTVFDPFWDLSLPIAKRGYPEVTLMDCMRLFTKEDVLDGDEKPTCCRCRGRKRCIKKFSIQRFPKILVLHLKRFSESRIRTSKLTTFVNFPLRDLDLREFASENTNHAVYNLYAVSNHSGTTMGGHYTAYCRSPGTGEWHTFNDSSVTPMSSSQVRTSDAYLLFYELASPPSRMHHHHHH |
| Components | PH1011-10 μg | PH1011-50 μg | PH1011-100 μg | PH1011-1 mg |
| Recombinant Human USP2 Protein (1 mg/mL) | 0.01 mL | 0.05 mL | 0.1 mL | 1 mL |
Store the components at -80 °C. | ||||