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Recombinant Human UBE2B, His

 
Catalog No.
P1227
重组人泛素偶联酶E2B, His( UBE2B, His)
组合的产品项目
规格价格库存 数量
10ug
¥ 886.00
Ship with 5-10 days
10X10ug
¥ 7,301.00
Ship with 5-10 days
50X10ug
¥ 16,382.00
Ship with 5-10 days

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A

背景

泛素结合酶 E2 B 属于泛素结合酶家族,由人类的 UBE2B 基因编码。泛素结合酶,也称为 E2 酶,更罕见地称为泛素载体酶,参与泛素化反应的第二步。在此反应中,E1 通过将分子共价连接至其活性位点半胱氨酸残基来激活泛素。然后,激活的泛素被转移到 E2 半胱氨酸上,然后 E2 分子通过结构保守的结合区域结合 E3。泛素化反应可以修饰蛋白质并调节蛋白质降解。 UBE2B 与 RAD18、UBR2 和 WAC 相互作用。其蛋白质序列与小鼠、大鼠和兔的同源物100%相同,这表明该酶在真核进化中高度保守。

Reference

[1]. Roest HP, van Klaveren J, de Wit J, et al. 1996. Cell, 86: 799-810.

[2]. Adegoke OA, Bedard N, Roest HP, et al. 2002. Am J Physiol Endocrinol Metab, 283: E482-9.

[3]. Kavakebi P, Hausott B, Tomasino A, et al. 2005. Mol Cell Neurosci, 29: 559-68.

[4]. Mulugeta Achame E, Wassenaar E, Hoogerbrugge JW, et al. 2010. BMC Genomics, 11: 367.

Information

Accession #P1227
Alternate NamesHR6B, Ubiquitin Carrier Protein B, Ubiquitin-protein Ligase B
SourceEscherichia coli.
Protein sequenceMHHHHHHAMG QLRSMSTPAR RRLMRDFKRL QEDPPVGVSG APSENNIMQW NAVIFGPEGT PFEDGTFKLV IEFSEEYPNK PPTVRFLSKM FHPNVYADGS ICLDILQNRW SPTYDVSSIL TSIQSLLDEP NPNSPANSQA AQLYQENKRE YEKRVSAIVE QSWNDS
M.Wt19 kDa
AppearanceSolution protein
Stability & Storage

Avoid repeated freeze-thaw cycles

It is recommended that hat the protein be aliquoted for optimal storage

6 months from date of receipt, -20 to -70°C as supplied.

ConcentrationSee label.
FormulationA 0.2 μm filtered concentrated solution in 50 mM HEPES, pH 7.6 with 125 mM NaCl, 10 % Glycerol, 5 % Trehalose, 1 mM DTT.
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.
Biological ActivityData is not available.
Shipping Condition/
HandlingCentrifuge the vial prior to opening.
UsageFor Research Use Only! Not to be used in humans.

质量控制

质量控制和DataSheet

批次:
    纯度 > 95 % by SDS-PAGE and HPLC analyses.
    Endotoxin: <1 EU per 1 ug of the protein by the LAL method.
  • Datasheet