Protease Inhibitor Cocktail for bacterial cell extracts (100 X in DMSO)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
在稳定的环境条件下，内源性蛋白质产生和降解处于平衡状态，因此其细胞水平是稳定的。
细胞粗提取物中含有许多能够降解提取物中蛋白质的内源性酶，如磷酸酶和蛋白酶。在体外从细胞和组织中提取蛋白质时，蛋白质产生几乎停止，降解增加。提高完整蛋白质产量的最佳方法是加入已知存在的那些酶的抑制剂。
Protease Inhibitor Cocktail for bacterial cell extracts (100 X in DMSO)用于细菌提取物以提高蛋白质的稳定性。该Cocktail起到抑制降解非磷酸化或磷酸化蛋白质底物的蛋白酶的作用。
该Protease Inhibitor Cocktail包含单独的组分，包括AEBSF、Bestatin、E-64和Pepstatin A，这些物质对半胱氨酸、氨肽酶、丝氨酸、天冬氨酸和金属蛋白酶具有广谱特异的抑制性。已经用细菌提取物对这种蛋白酶抑制剂Cocktail进行了优化和测试。该蛋白酶抑制剂Cocktail溶剂为DMSO，作为即用型溶液提供。
Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as bacterial cell extracts) before assaying.
Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.
| Tube | Catalog No. | Product Name | Summary | Targets | CAS Number | Smiles |
| A (100X in DMSO) | ||||||
| A2573 | AEBSF.HCl | 丝氨酸蛋白酶抑制剂 | Proteases|Serine Protease | 30827-99-7 | C1=CC(=CC=C1CCN)S(=O)(=O)F.Cl | |
| A2575 | Bestatin | 氨肽酶抑制剂 | Proteases|Aminopeptidase | 58970-76-6 | CC(C)CC(C(=O)O)NC(=O)C(C(CC1=CC=CC=C1)N)O | |
| A2576 | E-64 | 半胱氨酸蛋白酶（cysteine protease）抑制剂 | Proteases|Cathepsin | 66701-25-5 | CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O | |
| A2571 | Pepstatin A | 天冬氨酸蛋白酶抑制剂 | Proteases|Other Proteases | 26305-03-3 | CC(C)CC(C(CC(=O)O)O)NC(=O)C(C)NC(=O)CC(C(CC(C)C)NC(=O)C(C(C)C)NC(=O)C(C(C)C)NC(=O)CC(C)C)O | |
| B (100X in ddH2O) | ||||||
| EDTA, disodium salt, dihydrate | 金属蛋白酶抑制剂 | protease | 6381-92-6 | |||
| 储存在-20°C，稳定保持至少12个月。 | ||||||