Protease Inhibitor Cocktail(MS-SAFE, 50X in DMSO)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
粗细胞提取物含有许多能够降解提取物中的蛋白质的内源酶，例如磷酸酶和蛋白酶。当在体外从细胞和组织中提取蛋白质时，蛋白质产生被显著抑制，降解增加。提高完整蛋白质产量的最佳方法是加入已知存在的那些酶的抑制剂混合物进行蛋白酶的广谱抑制。
该蛋白酶抑制剂cocktail包含对半胱氨酸，丝氨酸，酸性蛋白酶和氨基肽酶具有广泛特异性的Aprotinin，Bestatin，E-64，Leupeptin等组分。本产品不含AEBSF，可避免由于AEBSF导致的质谱峰漂移而干扰质谱(Mass Spectrometry, MS)分析，因此使用本产品提取的蛋白样品可兼容质谱检测。该产品作为DMSO即用溶液提供，并单独额外提供EDTA以便选择性使用。
|
Tube |
Catalog No. |
Product Name |
Summary |
Targets |
CAS Number |
Smiles |
|
A (50X in DMSO) |
A2574 |
牛胰蛋白酶抑制剂 |
Proteases|Serine Protease |
9087-70-1 |
|
|
|
A2575 |
氨肽酶抑制剂 |
Proteases|Aminopeptidase |
58970-76-6 |
CC(C)CC(C(=O)O)NC(=O)C(C(CC1=CC=CC=C1)N)O |
||
|
A2576 |
半胱氨酸蛋白酶（cysteine protease）抑制剂 |
Proteases|Cathepsin |
66701-25-5 |
CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O |
||
|
A2570 |
丝氨酸和半胱氨酸蛋白酶抑制剂 |
Proteases|Serine Protease |
103476-89-7 |
O=C(C(C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H])([H])N([H])C(C([H])([H])[H])=O)N([H])C(C(N([H])C(C([H])=O)([H])C([H])([H])C([H])([H])C([H])([H])/N=C(N([H])[H])/N([H])[H])=O)([H])C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H] |
||
|
B (50X in ddH2O) |
|
EDTA, disodium salt, dihydrate |
金属蛋白酶抑制剂 |
Proteases | Metalloprotease |
6381-92-6 |
|
|
-20℃条件下储存一年。 |
||||||