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One-step TUNEL FITC Apoptosis Detection Kit

 
Catalog No.
K1133
细胞凋亡TUNEL检测(FITC)
组合的产品项目
规格价格库存 数量
20rxns
¥ 1,500.00
现货
50rxns
¥ 2,200.00
现货
100rxns
¥ 3,500.00
现货

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A05

Description

One-step TUNEL FITC Apoptosis Detection Kit可以用来检测组织或细胞在凋亡过程中细胞核DNA的断裂情况。细胞内的DNA内切酶在细胞凋亡时激活,切断核小体间的基因组DNA,DNA降解成180 - 200 bp或其它整数倍片段。基因组DNA断裂产生的3´-OH末端,在末端脱氧核苷酸转移酶(Terminal Deoxynucleotidyl Transferase, TdT)作用下在其末端添加上带标记的dUTP,从而可以在荧光显微镜或流式细胞仪下进行检测,Ex/Em=492 nm/517nm。

本试剂盒应用范围广泛,可以用于检测冷冻或石蜡切片中的细胞凋亡情况,也可以检测培养的贴壁细胞或悬浮细胞的凋亡情况。

Features

293A cells were divided into three groups: control group, Positive group using DNase I treatment, Apoptosis group using 10μM Camptothecin treatment. After 12 hours of treatment, TUNEL kit (K1133) was used to detect cell Apoptosis.

质量控制

质量控制和DataSheet

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相关生物数据

One-step TUNEL FITC Apoptosis Detection Kit
 

Components and Storage

Place product at -20 °C for storage; Among them, FITC-12-dUTP Labeling Mix, stored at -20 °C protected from light.