One-step TUNEL Cy3 Apoptosis Detection Kit
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
One-step TUNEL Cy3 Apoptosis Detection Kit可以用来检测组织或细胞在凋亡过程中细胞核DNA的断裂情况。细胞内的DNA内切酶在细胞凋亡时激活,切断核小体间的基因组DNA,DNA降解成180 - 200 bp或其它整数倍片段。基因组DNA断裂产生的3´-OH末端,在末端脱氧核苷酸转移酶(Terminal Deoxynucleotidyl Transferase, TdT)作用下在其末端添加上带标记的dUTP,从而可以在荧光显微镜或流式细胞仪下进行检测,Ex/Em=550nm/570nm。
本试剂盒应用范围广泛,可以用于检测冷冻或石蜡切片中的细胞凋亡情况,也可以检测培养的贴壁细胞或悬浮细胞的凋亡情况。
293A cells were divided into three groups: control group, Positive group using DNase I treatment, Apoptosis group using 10μM Camptothecin treatment. After 12 hours of treatment, TUNEL kit (K1134) was used to detect cell Apoptosis.