Nucleic and Cytoplasmic fluid extraction isolation kit
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
细胞核和细胞质的组分研究一直是细胞研究的重要方向，细胞内各组分特别是在细胞核的提取和分离后，不仅可以实现细胞内蛋白定位等研究，同时可实现核内蛋白的转录调控机制等方面研究。
本试剂盒提供了一种简单、便捷的从培养细胞和新鲜组织匀浆中提取胞质蛋白和细胞核内蛋白的方法，提取的蛋白可进行后续的Western blot等实验。
Western Blot analysis of cytoplasmic protein β-tubulin and intracellular protein Lamin A of 293T cells isolated by the kit listed above.
| Components | 50 rxns | 100 rxns | 200 rxns |
| Buffer A | 25 mL | 50 mL | 100 mL |
| Buffer B | 0.5 mL | 1 mL | 2 mL |
| Buffer C | 2.5 mL | 5 mL | 10 mL |
| Buffer D | 0.3 mL | 0.6 mL | 1.2 mL |
| Cell wash Buffer | 250 mL | 500 mL | 1 L |
| Protease Inhibitor Cocktail (EDTA-Free, 100X in DMSO)(K1010) | 0.3 mL | 0.6 mL | 1.2 mL |
Buffer A/C and Cell wash Buffer: Store at 4 °C; Others: Store at -20℃. | |||