Micro BCA Protein Assay Kit
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
BCA全称是Bicinchoninic Acid Assay，是基于双缩脲原理的蛋白定量方法。在碱性条件下，蛋白质肽键将硫酸铜中Cu2+还原成Cu+，随后BCA鳌合Cu+形成紫色复合物，强烈吸收562 nm波长的光。螯合物的颜色跟蛋白浓度有关，同时反应减少的Cu2+或Cu+获得量与溶液中存在的蛋白质的量成比例，因此可以通过检测蛋白吸光度值获取待测蛋白浓度。
本试剂盒专为低蛋白浓度样品的蛋白定量而设计，可测量稀释蛋白溶液（0.5~20 µg/mL）的总蛋白浓度。本试剂盒具有操作简便性、高稳定性、高灵敏度和高兼容性等特点，同多种离子和非离子型去污剂有较好的相容性。
| Components | K4102-500 T | K4102-2000 T |
| Reagent A | 40 mL | 160 mL |
| Reagent B | 38 mL | 150 mL |
| Reagent C | 2 mL | 6 mL |
| Bovine serum albumin (BSA) | 6 mg | 15 mg |
| BCA Protein Assay Reagent | 15 mL | 35 mL |
Store Reagent B at 4°C away from light and the other components at 4°C for a year. | ||