HyperChrom Resilient ARPA Agarose
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
HyperChrom Resilient ARPA Agarose 是一款用于抗体纯化的亲和层析介质，通过抗原与抗体之间特异性的相互作用进行分离。该层析介质用于处理高浓度单克隆抗体。
HyperChrom Resilient ARPA Agarose拥有高载量、高流速、耐碱的特性：(1)高流速、高动态结合载量缩短了处理时间。(2)修饰的耐碱rProtein A配基可耐受 0.5M NaOH 进行CIP。
HyperChrom Resilient ARPA Agarose层析介质参数
|
名称 |
描述 |
|
层析介质类型 |
亲和层析介质 |
|
配基 |
耐碱Protein A |
|
基架 |
高度交联琼脂糖 |
|
平均粒径 |
85 μm |
|
动态载量 |
≥60 mg人IgG/mL层析介质* |
|
推荐使用流速 |
90-500 cm/h |
|
最大流速 |
700 cm/h |
|
耐压 |
0.3 MPa |
|
使用温度 |
2-40℃ |
|
pH稳定性 ** |
2-14 |
|
化学耐受性 |
Protein A层析中常用的水溶性缓冲液 |
|
*动态载量的测量条件：装柱高度：10 cm，保留时间6 min，测试缓冲液：0.02 M NaH2PO4溶液，0.15 M NaCl, pH 7.4，当lgG穿透量达到10%时，单位介质体积 (mL) 的lgG上样量 (mg)。 **层析介质在40℃，pH 2-14环境下放置7天后，其理化性质和功能没有明显变化。 |
|
| Components | PC2004-25 mL | PC2004-100 mL |
| HyperChrom Resilient ARPA Agarose | 25 mL | 100 mL |
Store the components at 4℃ for 5 years. | ||