HyperTrap AR rProtein A/G 4FF Column
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
HyperTrap AR rProtein A/G 4FF Column是一款用于抗体纯化的亲和层析z预装柱，通过抗原与抗体之间特异性的相互作用进行分离，主要用于培养液、血清、腹水或杂交瘤细胞培养上清中的IgG的纯化，或含Fc片段的重组蛋白的纯化。
本产品中的HyperChrom AR rProtein A FF Agarose和HyperChrom Protein G 4FF Agarose共价连接到4%的高交联度、高流速琼脂糖上，并按照1:1比例混合。每毫升HyperChrom AR rProtein A/G Agarose 4FF Agaros可以结合约20 mg人IgG。
1. HyperTrap AR rProtein A/G 4FF Column预装柱参数
|
预装柱规格/名称 |
1 mL |
5 mL |
|
规格尺寸(IDxH) |
7.7 x 25 mm |
16 x 25 mm |
|
耐压 |
0.3 MPa |
0.3 MPa |
|
工作流速* |
1 mL/min |
1-3 mL/min |
|
使用温度 |
4 - 30℃ |
4 - 30℃ |
|
存储条件 |
Store the components at 4℃ for 5 years. |
|
|
*此流速为推荐的常用流速，预装柱中不同类型的层析填料的耐压性和使用流速会有区别，可参考对应层析填料的产品说明书。 |
||
2. HyperTrap AR rProtein A/G 4FF Column层析介质参数
|
名称 |
描述 |
|
层析介质类型 |
亲和层析介质 |
|
配基 |
rProtein A/G（E.coli 重组表达，1：1） |
|
基架 |
高度交联琼脂糖 |
|
平均粒径 |
90 μm |
|
动态载量 |
~20 mg人IgG/mL层析介质 |
|
推荐使用流速 |
50-400 cm/h |
|
耐压 |
0.3 MPa |
|
使用温度 |
4-30℃ |
|
pH稳定性 |
3-9 |