Hematoxylin and Eosin Staining Kit
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
苏木素伊红染色法(Hematoxylin and Eosin Staining),简称为HE染色法,是病理组织切片中应用最广泛的染色方法之一。苏木素(Hematoxylin),又称苏木精,是一种从洋苏木中提取得到的天然染料,被氧化后生成氧化苏木素,从而可以和媒染剂(三价的铝盐或铁盐等)形成有颜色的复合物。该复合物带正电荷,很容易与带负电荷的细胞核结合,从而将细胞核染成不同程度的蓝色或蓝紫色。
伊红(Eosin),又称曙红,是一种带红色荧光的酸性染料,在水中能水解成带负电荷的阴离子,能够与蛋白质氨基中带正电荷的阳离子结合从而将细胞质染成不同程度的红色或粉红色。
苏木素伊红染色后,细胞核呈蓝色,细胞质呈红色,两者形成鲜明对比,从而便于观察与分析。该染色方法应用广泛,可用于石蜡切片、冰冻切片以及细胞涂片等的染色。本试剂所含试剂均为工作液,可以直接使用。
Components | K1142-100 mL | K1142-500 mL |
Hematoxylin Stain Solution | 100 mL | 500 mL |
Eosin Staining Solution | 100 mL | 500 mL |
室温避光保存,有效期至少1年。 |