Fluorescein-12-dUTP
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Fluorescein -12-dUTP可用于直接酶标记DNA / cDNA,如PCR,随机引物DNA标记反应,引物延伸,Nick Translation等。它可以作为其天然对应物dTTP的替代物而被整合进入DNA/ cDNA。获得的染料标记的DNA / cDNA探针非常适合荧光杂交应用 - FISH或基于基因芯片的基因表达谱分析。与尿苷的C5位连接的优化接头确保了优化的底物特性和标记效率。推荐用于PCR和Nick Translation的Fluorescein-12-dUTP / dTTP比例:30-50%Fluorescein-12-dUTP / 70-50%dTTP。请注意:保护染料标记的dUTP免受光照,并在低光照条件下进行实验。染料标记的dUTP的最佳终浓度可能取决于实际应用和实验条件。为了获得最佳产品收率和高掺入率,建议对染料标记的dUTP / dTTP比率进行单独优化。
Fluorescein-12-dUTP可以作为以下酶的底物:
- Taq聚合酶
- phi29 DNA聚合酶
- DNA聚合酶I(全酶和Klenow片段)
- 末端转移酶
- 逆转录酶(例如AMV和M-MuLV)。
参考文献:
[1]. Wiegant et al. (2001) Probe Labeling and Fluorescence in situ Hybridization. Current Protocols in Cytometry 8:8.3.1.
[2]. Yie SM1, Xiao R, et, al. Progesterone regulates HLA-G gene expression through a novel progesterone response element. Hum Reprod. 2006 Oct;21(10):2538-44. Epub 2006 May 9.
Physical Appearance | Solution |
Storage | Store at -20°C or below |
M.Wt | 994.69 (free acid) |
Cas No. | 214154-36-6 |
Formula | C39H41N4O21P3 (free acid) |
Synonyms | Fluorescein-X-5-Aminoallyl-dUTP; 5/6-Fluorescein-X-5-aminoallyl-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt |
Solubility | Soluble in sterile water,pH 7.5 ±0.5 |
SDF | Download SDF |
Canonical SMILES | OC1=CC(O2)=C(C3(C4=C2C=C(O)C=C4)C5=C(C(O3)=O)C=C(C(NCCCCCC(NC/C([H])=C([H])/C(C(N6)=O)=CN(C6=O)[C@@]7([H])C[C@@](O)([H])[C@@](O7)([H])COP(O)(OP(O)(OP(O)(O)=O)=O)=O)=O)=O)C=C5)C=C1 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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