Biotin-11-dCTP
| 规格 | 价格 | 货期 | 数量 |
|---|---|---|---|
| 50uL(1 mM) | ￥2254.00 | 现货 |
特色产品
- 适用于流式细胞仪和荧光显微镜检测
- 准确区分活细胞/凋亡细胞/坏死细胞
- 用于免疫印迹和质谱分析等后续操作
- 适用于30 KDa-130 KDa大小的蛋白
- 对细胞无毒性
- 步骤更简单，无需有机溶剂
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰
- 优异的扩增效率、可重复性和稳定性
- 卓越的灵敏度和准确性，操作简单
- 优化的缓冲液和T7 RNA聚合酶混合物
- 20 μL反应体系可获得更高的RNA产量
产品描述
Biotin-11-dCTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of cytidine.
Recommended Biotin-11-dCTP/dCTP ratio for PCR and Nick Translation: 50% Biotin-11-dCTP/ 50% dCTP
Please note: The optimal final concentration of Biotin-11-dCTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Biotin-11-dCTP/dCTP ratio is recommended.
产品性质
| 物理外观 | Solution |
| CAS号 | 136632-30-9 |
| 分子式 | C28H44N7O16P3S (free acid) |
| 分子量 | 859.67 (free acid) |
| 化学名称 | γ-[N-(Biotin-6-amino-hexanoyl)]-5-propargylamino-2'-deoxycytidine-5'-triphosphate, Triethylammonium salt |
| SMILES | O=C1N([C@@H]2O[C@H](COP(OP(OP(O)(O)=O)(O)=O)(O)=O)[C@@H](O)C2)C=C(C#CCNC(CCCCCNC(CCCC[C@@]3([H])[C@](NC(N4)=O)([H])[C@]4([H])CS3)=O)=O)C(N)=N1 |
| 存储条件 | -20°C及以下 |
| 运输条件 | 干冰 |
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