EZ Cap™ Reagent GG (3' OMe)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
EZ Cap Reagent GG(3'OMe)是一种共转录加帽试剂,用于5'加帽mRNA的体外转录,产生Cap 1结构。
EZ Cap GG(3'OMe)含有在ARCA加帽的mRNA中常见的5'N7-甲基-3'-O-甲基鸟苷,而EZ Cap GG具有天然存在的5'N7-甲基鸟苷。
EZ Cap GG(3'OMe)需要GG起始序列,而EZ Cap GG(3'OMe)通常提供> 90%的具有天然存在的Cap 1结构的加帽产物。与EZ Cap AG不同,使用EZ Cap GG(3'OMe)可以产生与ARCA相当的转录性口吃和产量。
EZ Cap与野生型T7聚合酶兼容。建议使用5 mM EZ Cap和rNTPs。
模板设计是任何转录的重要组成部分。EZ Cap GG(3'OMe)应与特定的起始序列5'GG 3'一起使用。
最终加帽效率取决于EZ Cap Reagent、DNA模板和最终的mRNA序列。由于RNA长度和碱基组成而形成的二级结构可能影响最终的加帽效率。
Physical Appearance | Solution |
Storage | Store at -20°C or below |
M.Wt | 1175.6 (free acid) |
Formula | C33H45N15O25P4 (free acid) |
Synonyms | EZ Cap™ Reagent GG (3' OMe) for co-transcriptional capping of mRNA, m7(3'OMeG)(5')ppp(5')(2'OMeG)pG |
SDF | Download SDF |
Canonical SMILES | O=C1NC(N)=NC2=C1N=CN2C3C(O)C(O)C(COP([O-])(OC4C(OC)C(N5C(N=C(N)NC6=O)=C6N=C5)OC4COP([O-])(OP([O-])(OP([O-])(OCC7OC(N8C=[N+](C)C9=C8N=C(N)NC9=O)C(O)C7OC)=O)=O)=O)=O)O3.[NH4+].[NH4+].[NH4+] |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |