Cy3-UTP
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cy3-UTP (Cyanine 3-UTP) can replace UTP as a substrate for T7 RNA polymerase and generate labeled probes through in vitro transcription. The resulting assembled mRNA will emit orange fluorescence with the involvement of Cy3-UTPs. In addition, Cy3-labeled mRNA is readily visible under ultraviolet light without further staining after gel electrophoresis[1].
Probes generated by these methods are suitable for multicolor fluorescence analysis, specifically dual-color expression arrays in conjunction with cyanine 3-UTP .
Reference:
[1]Kim, H., Park, Y., & Lee, J. B. (2015). Self-assembled Messenger RNA Nanoparticles (mRNA-NPs) for Efficient Gene Expression. Scientific Reports, 5(1). doi:10.1038/srep12737
| Physical Appearance | Solution |
| Storage | Store at -20°C or below |
| M.Wt | 1151.98 (free acid) |
| Formula | C43H56N5O22P3S2(free acid) |
| Synonyms | Aminoallyl-UTP - Cy3;5-(3-Aminoallyl)-uridine-5'-triphosphate; labeled with Cy3;Triethylammonium salt;Cyanine 3-AA-UTP;Cyanine 3-Aminoallyluridine-5'-Triphosphate;Cyanine 3-UTP |
| Solubility | Soluble in H2O |
| Chemical Name | 2-((1E,3E)-3-(1-(6-(((E)-3-(1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(((hydroxy((hydroxy(phosphonooxy)phosphoryl)oxy)phosphoryl)oxy)methyl)tetrahydrofuran-2-yl)-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)allyl)amino)-6-oxohexyl)-3,3-dimethyl-5-sulfoindolin-2-ylid |
| SDF | Download SDF |
| Canonical SMILES | O=S(C1=CC=C([N+](CC)=C(C2(C)C)/C=C/C=C(C(C)(C)C3=C4C=CC(S(O)(=O)=O)=C3)/N4CCCCCC(NC/C=C/C5=CN([C@H]6[C@H](O)[C@H](O)[C@@H](COP(OP(O)(OP(O)(O)=O)=O)(O)=O)O6)C(NC5=O)=O)=O)C2=C1)([O-])=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
