EZ Cap™ Cas9 mRNA (5-moUTP)
Catalog No.
R1015
具有Cap1结构的Cas9 mRNA，使用5-moUTP修饰，提供更高的转录效率并抑制RNA介导的先天免疫激活
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Cas9/guide RNA (Cas9/gRNA)系统通常用于基因组编辑。将这些组分递送至细胞核后，RNA引导序列靶向目标位点，并使用Cas9蛋白进行DNA切割。 5moUTP修饰的Cas9 mRNA使用一种共转录加帽方法进行加帽，以高效率获得天然的Cap 1结构。相比于Cap 0结构，带有Cap 1结构的mRNA对哺乳动物系统更适合，并且具有更高的转录效率。 5moUTP修饰可减少先天免疫反应并增加mRNA活性。
| mRNA Length | 4527 nucleotides | ||
| Concentration | 1 mg/mL | ||
| Buffer | 1 mM Sodium Citrate, pH 6.4 | Storage | -40°C or below |
| General tips | 请将其于冰上溶解,并小心防止RNase污染降解。 尽可能避免反复冻融。 不要涡旋震荡。 首次使用时，将其轻柔离心并分成几份，可供单独使用。 使用不含RNase的试剂和耗材，使用适当的无RNase技术。 直至与转染试剂混合，才可加入含有血清的培养基中。 | ||
| Shipping Condition | 试用装：干冰运输。 | ||