Digoxigenin-11-dUTP

Digoxigenin-11-dUTP可以酶促掺入到DNA / cDNA中以替代其天然对应物dTTP。随后使用耦联辣根过氧化物酶（HRP），碱性磷酸酶（AP）或荧光染料的地高辛抗体检测得到的地高辛标记DNA / cDNA探针。通过连接在尿苷C5位上的11个原子的接头保证了最佳的底物特性和标记效率。

Digoxigenin-11-dUTP可以通过以下方法整合到DNA/cDNA中：

- 用Taq聚合酶进行PCR

- 用DNAse I / DNA聚合酶I进行Nick Translation

- 用Klenow exo-进行引物延伸

- 用末端脱氧核苷酸转移酶（TdT）标记寡核苷酸3'末端

- 用MMLV逆转录酶进行逆转录

Digoxigenin-11-dUTP可以通过以下方法整合到RNA中：

- 用末端脱氧核苷酸转移酶（TdT）标记3'末端

推荐的用于PCR和Nick Translation的Digoxigenin-11-dUTP / dTTP比例：35％地高辛-11-dUTP / 65％dTTP

请注意：Digoxigenin-11-dUTP的最佳终浓度取决于应用和实验条件。为获得最佳产品收率和高掺入率，建议单独优化Digoxigenin-11-dUTP / dTTP比率

参考文献：
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