Caspase-8 Colorimetric Assay Kit
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cysteine-dependent aspartate-directed proteases (Caspases) are a family of cysteine proteases that play important roles in apoptosis, necrosis, and inflammation. Sequential activation of caspases plays an important role in cell apoptosis. Caspase-8 is a caspase protein that cleaves and activates Caspase-3 and is involved in the programmed cell death induced by Fas and different apoptotic stimuli. Caspase-8 was detected in the insoluble fraction of the affected brain region in Huntington disease patients, which suggested it plays an important role in neurodegenerative diseases.
Caspase-8 Colorimetric Assay Kit provides a convenient and simple way for detecting the IETD-dependent caspase activity. When cleavage of IETD from the labeled substrate IETD-p-nitroaniline (IETD-pNA) by Caspase-8 or related caspases, the free pNA light emission can be quantified by using a microtiter plate reader or a spectrophotometer at 405 or 400 nm. Comparison of the absorbance of free pNA from an apoptotic sample with an uninduced control determines the fold increase in Caspase-8 activity.
| Features | Fast and convenient Simple one-step procedure; takes 1-2 hours. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-8 activity. |
| Shipping | Gel pack |
| Storage Conditions | Store at -20°C. |
Related Biological Data
Related Biological Data
Related Biological Data
| Species reactivity | Mammalian |
| Applications | Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. |
| Kit components | Cell Lysis Buffer 2X Reaction Buffer IETD-pNA (4 mM) DTT (1 M) Dilution Buffer |