Caspase-1 Colorimetric Assay Kit
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
含半胱氨酸的天冬氨酸蛋白水解酶(Caspase)是一个半胱氨酸蛋白酶家族,在细胞凋亡、坏死和炎症中起重要作用。Caspase的连续激活在细胞凋亡中起重要作用。Caspase-1,又称为IL-1β转换酶(IL-1β-converting enzyme, ICE),是固有免疫中的关键调节因子。激活的Caspase-1可以剪切并激活促炎细胞因子IL-1β和IL-18,从而调控炎症反应。Caspase-1可以诱导细胞凋亡或细胞焦亡,最终引起细胞死亡。
Caspase-1 Colorimetric Assay Kit为检测YVAD依赖性Caspase活性提供了一种方便且简单的方法。当Caspase-1 或相关的半胱天冬酶从标记的底物YVAD-p-硝基苯胺(YVAD-pNA)上裂解YVAD时,可以使用酶标仪或分光光度计在405 nm或400 nm处测量游离pNA的光发射。将凋亡样品中游离pNA的吸光度与未诱导的对照进行比较,确定 Caspase-1活性的增加倍数。
简单的一步反应;1-2h即可检测
实验操作方便快速
通过比较实验组和对照组的pNA吸光度强度测定caspase-1活性变化
Components | K2011-25 25 Assays |
K2011-100 100 Assays |
K2011-200 200 Assays |
K2011-400 400 Assays |
Cell Lysis Buffer | 25 mL | 100 mL | 100 mL | 100 mL |
2X Reaction Buffer | 2 mL | 4X 2 mL | 16 mL | 32 mL |
YVAD- pNA (4 mM) | 125 µL | 500 µL | 2X 0.5 mL | 2X 1 mL |
DTT (1 M) | 100 µL | 400 µL | 400 µL | 400 µL |
-20°C保存。 |