Biotin-11-dUTP
| 规格 | 价格 | 货期 | 数量 |
|---|---|---|---|
| 10uL(5 mM) | ￥2727.00 | 现货 | |
| 200uL(5 mM) | ￥7372.00 | 现货 |
特色产品
- 适用于流式细胞仪和荧光显微镜检测
- 准确区分活细胞/凋亡细胞/坏死细胞
- 用于免疫印迹和质谱分析等后续操作
- 适用于30 KDa-130 KDa大小的蛋白
- 对细胞无毒性
- 步骤更简单，无需有机溶剂
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰
- 优异的扩增效率、可重复性和稳定性
- 卓越的灵敏度和准确性，操作简单
- 优化的缓冲液和T7 RNA聚合酶混合物
- 20 μL反应体系可获得更高的RNA产量
产品描述
Biotin-11-dUTP, a biotinylated deoxynucleotide triphosphate analogue, can be enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart deoxythymidine triphosphate (dTTP). The resulting biotin-labeled DNA/cDNA probes are then detected with streptavidin-horseradish peroxidase (HRP) conjugates, alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Biotin-11-dUTP is commonly used for PCR, nick translation, as well as reverse transcription. It is also suitable for apoptosis assays.
References:
1. Choi JH, Kim SY, Kim SK, et al. An Integrated Approach for Analysis of the DNA Damage Response in Mammalian Cells. Journal of Biological Chemistry, 2015, 290(48): 28812-28821.
2. Day PJ, Bevan IS, Gurney SJ, et al. Synthesis in vitro and application of biotinylated DNA probes for human papilloma virus type 16 by utilizing the polymerase chain reaction. Biochemical Journal, 1990, 267(1): 119-123.
产品性质
| 物理外观 | Solution |
| CAS号 | 1221498-88-9 |
| 分子式 | C28H44Li4N7O16P3S |
| 分子量 | 887.44 |
| 化学名称 | lithium (5-(4-amino-2-oxo-5-((E)-3-(6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)prop-1-en-1-yl)-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl triphosphate |
| 溶解度 | ≥100.7 mg/mL in H2O; ≥46.3 mg/mL in EtOH; ≥95.6 mg/mL in DMSO |
| SMILES | O=C1N[C@]2([H])[C@]([C@H](CCCCC(NCCCCCC(NC/C=C/C3=CN(C(NC3N)=O)C4OC(COP(OP(OP([O-])([O-])=O)([O-])=O)([O-])=O)C(C4)O)=O)=O)SC2)([H])N1.[Li+].[Li+].[Li+].[Li+] |
| 存储条件 | -20°C及以下 |
| 运输条件 | 干冰 |
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