Annexin V-HF488/7-AAD Apoptosis Kit
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Annexin V是一种Ca2+依赖性磷脂结合蛋白，可与磷脂酰丝氨酸（Phosphatidylserine, PS）特异结合。PS正常位于细胞膜脂质双层的内侧，但在细胞凋亡早期PS可从细胞膜的内侧翻转到细胞膜的外表面，暴露在细胞外环境中。Annexin V对PS具有高亲和力，对Annexin V进行荧光素（HF488）标记，以带有标记的Annexin V作为荧光探针，利用流式细胞仪或荧光显微镜可检测细胞凋亡的发生，染色过程仅需10-20分钟。7-AAD可与双链DNA特异性结合产生红色荧光，正常情况下无法透过细胞膜。但在凋亡晚期的细胞和死细胞，7-AAD能够透过细胞膜而使细胞核染色。当同时进行Annexin V-HF488和7-AAD染色时可区分处于不同凋亡时期的细胞及死细胞。
图1：本试剂盒检测细胞凋亡验证图
| Features | 1. 快速：在10-20分钟内完成染色。 |
| Components | K2254-20 Assays | K2254-50 Assays | K2254-100 Assays |
| Annexin V-HF488 | 100 μL | 250 μL | 500 μL |
| 10X Binding Buffer | 2.8 mL | 5.5 mL | 11 mL |
| 7-AAD | 100 μL | 250 μL | 500 μL |
Store the kit at 2-8°C stable for 6 months. Annexin V-HF488 and 7-AAD should be stored away from light. Do not freeze Annexin V-HF488. | |||