2X Hyperfusion plus master mix
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
我们的产品2X Hyperfusion plus master mix能够为大部分PCR应用同时提供高保真性,强大的扩增能力和更快的延伸速度。Hyperfusion plus DNA 聚合酶由Pyrococcus样校正聚合酶与DNA结合结构域融合形成。其独特的结构,一种新型Pyrococcus样酶与具有增强合成能力的结构域融合,提高了保真度和延伸速度。高保真度使得hyperfusion plus DNA 聚合酶成为分子克隆或其他后续应用的较优选择。Hyperfusion plus DNA 聚合酶是具有最高的保真度的DNA聚合酶中的一个,其错误率比Taq DNA聚合酶低50倍,比Pyrococcus Furious DNA聚合酶低6倍。
Hyperfusion plus DNA 聚合酶具有5’→3'聚合酶活性和3'→5'外切酶活性。它会在扩增产物两端产生平端,而没有A突出(A突出通常出现在用Taq聚合酶扩增的产物中)。在实验中我们的Hyperfusion plus聚合酶能够扩增长达20.1 kb 的基因组片段。在PCR反应中,Hyperfusion plus DNA聚合酶的延伸速率约为15-30 sec/kb(取决于基因的复杂性)。
2X Hyperfusion plus master mix是一种即用型2×预混液,其中包含聚合酶、dNTP,和已经优化的缓冲系统。
Component | K1119-1 mL | K1119-5x1 mL | K1119-20x1 mL | K1119-50x1 mL | K1119-100x1 mL |
2X Hyperfusion plus master mix | 1 mL | 5 mL | 20 mL | 50 mL | 100 mL |
Store the components at -20°C for 12-24 months. |