T4 Gene 32 Protein(T4gp32)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
T4噬菌体基因32编码蛋白(T4 gene 32 protein, T4 GP32 Protein)是一种单链 DNA（ssDNA）结合蛋白，为T4噬菌体DNA复制和修复所必需。它协调性地结合并稳定瞬时形成的ssDNA区域，在T4 噬菌体DNA复制过程中起着重要的结构性作用。该蛋白也被广泛地用于稳定和标记ssDNA区域，以便用电子显微镜观察细胞内DNA的结构。最新报道表明，T4 gene 32 protein可以改善限制性内切酶的酶切、提高RT-PCR过程中逆转录（RT）反应的产量和效率、增强T4 DNA聚合酶活性，以及提高PCR产物的产量。
本产品主要用于在RT-PCR过程中，增加反转录的产量和延伸能力，以及土壤样品进行PCR时，增加目的片段的产量和特异性，以及稳定和标记ssDNA结构。
|
产品名字 |
T4 Gene 32 Protein |
|
分子量 |
~33,506 Da |
|
来源 |
An E. coli strain carrying a plasmid that overexpresses the gene 32 protein of T4 phage |
|
浓度 |
10 mg/mL |
|
反应条件 |
37℃ (Optimal reaction temperature) |
|
贮存溶液 |
20 mM Tris-HCl, 100 mM NaCl, 0.5 mM DTT, 1 mM EDTA, 50% Glycerol (pH 8, 25°C) |
|
储存条件 |
-20℃ |
|
储存时间 |
2 years |
|
运输条件 |
Dry ice |