Rhodamine 123 (chloride)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Rhodamine 123 (chloride),即罗丹明123,是一种可对活细胞线粒体染色的细胞染色试剂。罗丹明123可透过细胞膜且在活细胞的线粒体内聚集,并发出黄绿色荧光,对细胞没有任何毒性。罗丹明123广泛用作检测线粒体膜电位[1],也常用于细胞凋亡检测[2]。由于细胞内ATP的量与罗丹明123的荧光强度之间有相关性,此荧光染料被应用于检测细胞内的ATP。罗丹明123还用于癌症研究,例如前列腺癌[3,4]。
罗丹明123的最大激发波长为507nm,最大发射波长为529nm。在荧光显微镜下观察,呈现黄绿色荧光。
References:
[1] Baracca A, Sgarbi G, Solaini G, et al. Rhodamine 123 as a probe of mitochondrial membrane potential: evaluation of proton flux through F(0) during ATP synthesis. Biochim Biophys Acta. 2003 Sep 30;1606(1-3):137-46.
[2] Ronot X, Benel L, Adolphe M, et al. Mitochondrial analysis in living cells: the use of rhodamine 123 and flow cytometry. Biol Cell. 1986;57(1):1-7. Review.
[3] Arcadi JA, Narayan KS, Techy G, et al. Studies of rhodamine-123: effect on rat prostate cancer and human prostate cancer cells in vitro. J Surg Oncol. 1995 Jun;59(2):86-92; discussion 92-3.
[4] Jones LW, Narayan KS, Shapiro CE, et al. Rhodamine-123: therapy for hormone refractory prostate cancer, a phase I clinical trial. J Chemother. 2005 Aug;17(4):435-40.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 380.8 |
Cas No. | 62669-70-9 |
Formula | C21H17N2O3·Cl |
Synonyms | R-22420,R-302,RH123 |
Solubility | ≥10.65 mg/mL in EtOH with ultrasonic; ≥2.25 mg/mL in H2O with ultrasonic; ≥20.5 mg/mL in DMSO with ultrasonic |
Chemical Name | 3,6-diamino-9-[2-(methoxycarbonyl)phenyl]-xanthylium, monochloride |
SDF | Download SDF |
Canonical SMILES | NC1=CC2=[O+]C3=C(C(C4=CC=CC=C4C(OC)=O)=C2C=C1)C=CC(N)=C3.[Cl-] |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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