Recombinant Murine Fatty-acid-binding Protein 1
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
脂肪酸结合蛋白(FABP)是脂肪酸和其他亲脂物质(如二十烷基类和维甲酸)的载体蛋白家族。这些蛋白质被认为有助于脂肪酸在胞外膜和胞内膜之间的转移。脂肪酸结合蛋白1基因编码的脂肪酸结合蛋白1，又称肝型脂肪酸结合蛋白，是脂肪酸结合蛋白家族的一员，是一种高度保守的小分子细胞质蛋白。此外，FABP1还与游离脂肪酸及其辅酶A衍生物、胆红素和细胞质中的其他一些小分子结合。此外，它还可能参与细胞内的脂质运输。通过氨基酸序列比较，小鼠FABP1的氨基酸同源性分别为84%和94%。与人和大鼠FABP1的序列同源性。
Reference
1. Chmurzynska A. 2006. J Appl Genet. 47:39-48
2. Weisiger RA. 2002. Mol Cell Biochem. 239:35-43
3. Chen SH, Van Tuinen P, Ledbetter DH, et al. 1986. Somat Cell Mol Genet. 12:303-6
4. Weickert MO, Loeffelholz CV, Roden M, et al. 2007. Am J Physiol Endocrinol Metab. 293:E1078-84
5. Bassuk JA, Tsichlis PN, Sorof S. 1987. Proc Natl Acad Sci U S A. 84:7547-51
6. He Y, Yang X, Wang H, et al. 2007. Biochemistry. 46:12543-56.
| Gene ID | 14080 |
| Accession # | P12710 |
| Alternate Names | 14 kDa Selenium-binding Protein, Fatty Acid-binding Protein 1, L-FABP |
| Source | Escherichia coli. |
| M.Wt | Approximately 14.2 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids. |
| AA Sequence | MNFSGKYQLQ SQENFEPFMK AIGLPEDLIQ KGKDIKGVSE IVHEGKKIKL TITYGPKVVR NEFTLGEECE LETMTGEKVK AVVKLEGDNK MVTTFKGIKS VTELNGDTIT NTMTLGDIVY KRVSKRI |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 2 % trehalose. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Fully biologically active when compared to standard. The binding affinity of rMuFABP1 for the synthetic ligand cis-parinaric acid has been measured by fluorescence titration. Half maximal fluorescence of 2.5 μM rMuFABP1 is achieved with approximately 5 μM cis-paranaric acid. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
质量控制和DataSheet
- 批次:
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纯度 > 95 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rMuFABP1 as determined by LAL method.