Merbromin

Merbromin（汞溴红，CAS 号 129-16-8）是含汞荧光染料类化合物，核心生物活性为抗菌、防腐及荧光标记，兼具抑制革兰氏阳性菌 / 阴性菌生长与蛋白质结合的特性，作用机制包括破坏微生物细胞膜、与蛋白质（如胰蛋白酶）发生静态荧光猝灭结合（结合常数约 2700~3000 M⁻¹）；作为割伤和擦伤的局部消毒剂，推荐使用 2% 浓度溶液局部涂抹；作为生物染料时，使用对象为蛋白质（如牛源胰蛋白酶），常用方法包括稳态荧光光谱、时间分辨荧光光谱、荧光各向异性及吸收光谱分析，用于研究蛋白质 - 染料相互作用，推荐剂量为染料浓度约 10⁻⁶ M，搭配 10⁻⁴~10⁻³ g/ml 的胰蛋白酶浓度，在 pH 3~8 的柠檬酸盐缓冲液中进行，可通过荧光参数变化测定结合常数、微粘度及结合比例等指标。

参考文献：

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