Polyethylenimine Linear(PEI), MW40000
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
线性化聚乙烯亚胺PEI (分子量40000)是一种高电荷阳离子聚合物，非常容易与DNA或其他带负电荷生物大分子相互结合，使之成为常见的行之有效的细胞转染试剂。原理上PEI能将DNA缩合成带正电荷的复合物，黏附在带有负电荷的细胞表面残基上，之后通过胞吞作用进入细胞。
该转染试剂作为一种瞬时转染试剂，具有效率高、成本低、相对稳定等优点，试剂能与含血清的培养基兼容，目前已经验证广泛适用于多种常见细胞系包括HEK-293、HEK293T、CHO-K1、HepG2和Hela细胞的转染等。在HEK293和CHO细胞表达系统中，PEI在不同规模(从96孔板到100L生物反应器)的转染中均能提供很好的转染效果。
| Components | 4 mL (2.5 mg/mL) | 8 mL (2.5 mg/mL) |
| PEI-2.5 mg/mL | 4 mL | 8 mL |
| 2X Transfection Buffer | 3 mL | 6 mL |
Store the components at 4°C. | ||