mRNA Magnetic Purification Kit
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
mRNA Magnetic Purification Kit是从总RNA或体外转录体系中分离纯化mRNA的试剂盒,其中采用特殊设计的单分散超顺磁珠,表面共价偶联寡聚dT序列。基于寡聚dT序列和mRNA的poly A尾之间的碱基配对实现高纯度mRNA分离。试剂盒整体操作简单快速,提取的mRNA可将结合的dT序列作为第一链反应引物合成cDNA,或者将mRNA从磁珠洗脱用于各类下游分子实验,如RT-PCR、RPA(核糖核酸酶保护试验)、文库构建、Northern Blot分析、二代测序等。
Components | 2 mL | 5 mL |
Oligo (dT) 25 beads (10 mg/mL) | 2*1 mL | 5*1 mL |
Binding Buffer | 5 mL | 3*5 mL |
Washing Buffer | 5 mL | 3*5 mL |
Tris-HCl Buffer | 1 mL | 3*1 mL |
RNase-free water | 2*1 mL | 5*1 mL |
Store at 4 °C, no freezing! |