JC-10 Mitochondrial Membrane Potential Assay Kit
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
JC-10线粒体膜电位检测试剂盒（JC-10 Mitochondrial Membrane Potential Assay Kit）是一种通过荧光探针JC-10检测细胞内线粒体膜电位变化的试剂盒。本试剂盒可以用于细胞线粒体、组织线粒体或纯化的线粒体的膜电位检测。
ΔΨm，即线粒体膜电位（Mitochondrial Membrane Potential），是反映线粒体功能的一个重要参数。同时线粒体膜电位下降是细胞早期凋亡的重要标志，通过检测线粒体膜电位变化可以得知细胞的凋亡情况。JC-10是一种广泛使用的线粒体膜电位检测探针。在线粒体膜电位较高时，JC-10聚集在线粒体基质中并形成聚合物，发出明亮的红色荧光；当线粒体膜电位下降时，JC-10不能聚集在线粒体中，并从聚合物转变成单体，此时JC-10为绿色荧光。通过红/绿荧光的相对比例就可以判断线粒体膜电位下降的情况。相比于另一种常用的线粒体荧光探针JC-1，JC-10的水溶性更好，同时检测灵敏度更高。
本试剂盒提供CCCP作为诱导线粒体膜电位下降的阳性对照，对于大多数细胞，经CCCP处理后，线粒体膜电位基本完全消失。对于6孔板，本试剂盒可检测100个样品；对于12孔板，本试剂盒可检测200个样品。
图1：本试剂盒检测线粒体膜电位的效果图
| Components | K2001-100 T |
| JC-10(200X) | 5 x 100 µL |
| ddH2O | 90 mL |
| Staining Buffer (5X) | 80 mL |
| CCCP (10 mM) | 20 µL |
This kit should be stored at -20°C, stable for 1 year. JC-10 (200X) and CCCP (10 mM) should be stored at -20°C away from light, and avoid repeated freeze/thaw cycles. | |