InstaBlue Protein Stain Solution
![mRNA synthesis](/media/diy/images/page/figure1-mrna.png)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
![Tyramine Signal Amplification (TSA)](/media/diy/images/page/figure2-01.png)
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
![screening library](/media/diy/images/page/figure3-01.png)
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
![Cell Counting Kit-8 (CCK-8)](/media/diy/images/page/CCK-8.jpg)
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
![SYBR Safe DNA Gel Stain](/media/diy/images/page/SYBR Safe DNA Gel Stain.png)
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
![Inhibitor Cocktails](/media/diy/images/page/Inhibitor Cocktails.jpg)
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
InstaBlue protein stain solution是一种即用型考马斯亮蓝蛋白染色液,用于聚丙烯酰胺凝胶染色,采用专利配方,不需要清洗、固定、煮沸、脱色等复杂操作,直接用该染液染色2min即可看到蛋白条带,随着染色时间增加,条带会更清晰,而背景很干净,无需固定、洗涤和脱色处理。InstaBlue染色液配方使用安全,易于处理。
该染色液使用非常方便,一步法即可完成染色操作,大大缩短染色需要的时间,而一些预制和传统自制的考马斯R-250染色液可能需要3小时或更长时间才能完成染色操作。
而且,该染色液具有极高的敏感性,过夜染色时,低至5ng的蛋白条带也可以被检测到,这是由于该染色液对蛋白进行特异性染色,具有干净的背景,从而提高了染色的信号/噪音比。InstaBlue是无毒的,并且由于其无甲醇成分,不会使凝胶收缩。每瓶InstaBlue至少可以染40块小胶,每块小胶只需25ml,使您可以使用更少的蛋白染色液。而自制染色液和一些预混合溶液,每块凝胶需要约50-100毫升的染色液。
- 1. Huan Li, Chao Song, et al. "Transgelin Promotes Glioblastoma Stem Cell Hypoxic Responses and Maintenance Through p53 Acetylation." Adv Sci (Weinh). 2023 Dec 12:e2305620. PMID: 38087889
- 2. Jiuxiao Ruan, Huhui Chen, et al. "Brassinosteroids repress the seed maturation program during the seed-to-seedling transition." Plant Physiol. 2021 May 27;186(1):534-548. PMID: 33620498
- 3. Luling He, Qin Gong, et al. "Proteomic changes in rat kidney injured by adenine and the regulation of anemoside B4." Journal of Chinese Pharmaceutical Sciences. January 2019.
化学结构
![InstaBlue Protein Stain Solution](http://www.apexbt.com//media/diy/images/struct/B8226.png)
相关生物数据
![InstaBlue Protein Stain Solution InstaBlue Protein Stain Solution](http://www.apexbt.com/media/diy/images/wb/B8226_1.jpg)
相关生物数据
![InstaBlue Protein Stain Solution InstaBlue Protein Stain Solution](http://www.apexbt.com/media/diy/images/wb/B8226_2.jpg)
相关生物数据
![InstaBlue Protein Stain Solution InstaBlue Protein Stain Solution](http://www.apexbt.com/media/diy/images/wb/B8226_3.jpg)
相关生物数据
![InstaBlue Protein Stain Solution InstaBlue Protein Stain Solution](http://www.apexbt.com/media/diy/images/wb/B8226_4.jpg)
相关生物数据
![InstaBlue Protein Stain Solution InstaBlue Protein Stain Solution](http://www.apexbt.com/media/diy/images/wb/B8226_5.jpg)
超快染色——2min即可显色;
一步法——不需要洗涤、固定、微波或脱色;
高灵敏度——可检测到5ng的蛋白条带;
背景清晰——高信/噪比;
灵活——不会过度着色;
可靠——批量一致性;
经济——每块胶只需25ml染色液;
成分安全——无毒,无需通风橱或溶剂处理;
无甲醇——不会出现凝胶收缩或蛋白甲基化;
无乙酸——不会出现蛋白乙酰化;
质谱兼容性——没有残留的甲基化或乙酰化