HyperScript™ III First-Strand cDNA Synthesis SuperMix
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
HyperScript™ III Reverse Transcriptase是在M-MLV Reverse Transcriptase基础上经过基因工程改造后获得的第三代全新逆转录酶。相较野生型和其他版本而言,HyperScript™ III Reverse Transcriptase降低了RNase H活性提高了热稳定性和保真度。进而具有更高的cDNA合成产量和长度,更高的对于高GC含量RNA的逆转录效率和整体更好的性能等特点。HyperScript™ III Reverse Transcriptase增强了与模板的亲和力,适合少量模板以及低拷贝基因的逆转录,可扩增长达12.3 kb的cDNA。
本试剂盒包含合成第一链cDNA所需的所有组分,并提供Random Primers和Oligo (dT)23VN 两种cDNA合成引物。可根据实验需要选择Random Primers、Oligo(dT)23VN或基因特异性引物作为逆转录引物,合成的第一链cDNA产物可用于后续的PCR扩增、qPCR反应等实验。
Components | 50 rxn (20 μL reaction) | 100 rxn (20 μL reaction) |
RNase Free ddH2O | 1 mL | 2 X 1 mL |
5 X HyperScript™ Ⅲ SuperMix | 200 μL | 400 μL |
Random Primers (50 μM) | 50 μL | 100 μL |
Oligo (dT)23VN (50 uM) | 50 μL | 100 μL |
5 X HyperScript™ Ⅲ control Mix | 20 μL | 40 μL |
Store the components at -20℃ for 2 years. |