Hoechst 33342/PI Double Staining Kit
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Hoechst 33342/PI双染试剂盒(Hoechst 33342/PI Double Staining Kit)是一种可以快速检测细胞凋亡与坏死的试剂盒。本试剂盒提供两种染料,Hoechst 33342和碘化丙啶PI。Hoechst 33342可以穿透细胞膜,将正常细胞染成弱蓝色。当细胞凋亡时,染色体会固缩。此时Hoechst 33342可以将凋亡细胞染成强蓝色。PI不能透过细胞膜,不能将正常细胞或凋亡细胞染上红色;而对于坏死细胞(其细胞膜丧失完整性),PI可以将其染成红色。当使用这两种染料双染时,正常细胞为弱蓝色+弱红色荧光,凋亡细胞为强蓝色+弱红色荧光,坏死细胞为强蓝色+强红色荧光。
本试剂盒使用方便,且染色快速,仅需20-30 min即可完成染色。
图1:本试剂盒检测示意图
正常细胞(Control)主要被Hoechst 33342染成弱蓝色;当细胞被诱导发生凋亡时,凋亡细胞(Apoptosis)可被Hoechst 33342染成强蓝色,坏死细胞被PI染成强红色
Components | K2237-100 T | K2237-500 T |
Hoechst 33342染色液 | 250 µL | 1.25 mL |
PI染色液 | 250 µL | 1.25 mL |
染色缓冲液 | 100 mL | 5 x 100 mL |
-20°C保存,1年有效。其中Hoechst 33342染色液和PI染色液需要避光保存。 |