Fluorescein TSA Fluorescence System Kit
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
APExBIO公司的酪胺信号放大(TSA)系统可用于检测荧光免疫细胞化学(ICC)、免疫组织化学(IHC)和原位杂交(ISH)技术中的低丰度靶点,可将信号灵敏度提高100倍。
TSA荧光试剂盒使用辣根过氧化物酶(HRP)直接催化固定化酶周围的荧光基团共价沉积。该标记过程迅速(少于10分钟), 沉积标记可直接在标准或共聚焦显微镜下观察。 TSA荧光试剂盒也可以与抗荧光组合使用。
TSA技术可用于酶缀合物和合适的生色底物的明场显微镜检查。与普通实验相比,使用TSA试剂可显著提高信号灵敏度,同时保持稳定的特异性和分辨率。此外,TSA试剂可以显着减少一抗或探针的消耗量。本试剂盒(K1050)荧光标记信号为fluorescein,可在激发和发射波长(494nm/517nm)下显微检测信号。
酪胺信号放大系统原理简图
使用常规方法用一抗和二抗标记细胞或组织样品。与二抗缀合的辣根过氧化物酶催化标记的酪酰胺转化为反应性基团, 之后酪胺基团与附近的酪氨酸残基共价结合,提供高密度标记。
Complete Kit | 100-300 slides | 200-600 slides |
1X Amplification Diluent | 30 mL | 60 mL |
Fluorescein Tyramide (dry, dissolve in 60 μL DMSO) | 1 tube | 2 tubes |
Blocking Reagent | 6 g | 12 g |
Store Fluorescein Tyramide in the dark at -20°C for 1 year. Keep 1X Amplification Diluent and Blocking Reagent at 4°C for 1 year. |