Calpain Activity Fluorometric Assay Kit
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Apoptosis is one of the biological processes that activation of calpain is implicated in. Cell membrane and Ca2+ is required for calpainactivation. The activated calpain is entered into cytosol. The Calpain Activity Assay Kit is a convenient way of measuring calpain activity with optimized buffers and reagents. The Extraction Buffer extracts cytosolic proteins with contaminations from cell membrane and lysosome proteases. It also protects the cell from atuo-activation of calpain during extraction process. Thus, the kit only detects activated calpain in cytosol following treatment of cells with chemicals or drugs inducers. The fluorometric assay is based on the detection of cleavage of calpain substrate Ac-LLY-AFC. Ac-LLY-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by calpain, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorescence.
| Features | Simple one-step procedure; takes only 2 hours. Fast and convenient. The kit contains both positive and negative controls. Active calpain and calpain inhibitor and antibodies are also available separately. |
| Shipping | Dry ice |
| Storage Conditions | Store at -70°C. |
Related Biological Data
Related Biological Data
Related Biological Data
| Species reactivity | Mammalian |
| Applications | The kit provides a simple and convenient means for analyzing calpain activity in apoptotic and other samples. The assay is based on fluorometric detection of the cleavage of calpain substrate at 505 nm using a fluorometer or a fluorecence plate reader. |
| Kit components | Extraction Buffer 10X Reaction Buffer Calpain Substrate Ac-LLY-AFC Active Calpain I (Positive Control) Calpain Inhibitor Z-LLY-FMK |